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20 March 1996 <br /> AGE-NC Project No 95-0130 <br /> Page 20 of 23 <br /> organic chemicals into biomass for reproduction and growth Typically, the light-end hydrocarbons <br />' are degraded more rapidly, and heavier hydrocarbon compound are degraded more slowly <br />' 7 3 2 NUTRIENT REQUIREMENTS AND TOXICITY <br /> The microbes require certain essential nutrients that are sometimes available in ground water If <br />' supplemental nutrients are required, which commonly consist of nitrogen and phosphorus as <br /> ammonium phosphate in liquid phase, the quantity is usually very low However, a Waste Discharge <br /> Permit is required by the Regional Water Quality Control Board for the injection of nutrients into <br />' ground water <br /> Typically, microbes utilized in bioremediation are aerobic and usually require additional oxygen to <br />' supplement dissolved oxygen in ground water Generally, several air spargmg or in-well aeration <br /> sources are sufficient to provide the necessary oxygen However, in some cases the addition of <br /> hydrogen peroxide is necessary <br /> 7 3 3 FEASIBILITY <br /> Althoughn <br /> o bioremediation pilot test have been performed at the site, lighter petroleum <br />' hydrocarbons, such as gasoline compounds are biodegraded more readily than longer-chain (heavier) <br /> hydrocarbons, such as diesel fuel compounds Based on the fact that hydrocarbon-impacted soil at <br /> the site consists of lighter petroleum hydrocarbons, an in-situ treatment with the addition of sufficient <br />' oxygen supplement appears to be a feasible treatment method The additional oxygen could be <br /> supplied through an air sparging system or by adding an oxygen-laden solution such as hydrogen <br /> peroxide <br /> I <br /> 7 3 4 DURATION <br /> Cleanup durations for in-situ bioremediation will vary, depending upon specific site characteristics <br /> However, 12 to 24 months is generally considered an achievable remediation time Comparing <br /> dissolved hydrocarbon concentrations at the Top Filling Station with similar sites, a cleanup period <br /> of 24 months is likely <br /> 7 3 5 ESTIMATED COST OF IN-SITU BIOREMEDATION OF GROUND WATER <br /> Bioremediation of ground water can be a very cost-effective method of treatment With little on-site <br /> equipment required beyond the initial inoculation, the cost for in-situ bioremediation at the site would <br />