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BCM rroject No. 14-16243 Sampling Areas The waste water within the septic system holding tanks was sampled for the presence of any hazardous materials to be classified for proper disposal prior to being pumped out and disposed of at a local water treatment facility. Samplinp_ Methodolo>?y The waste water from the septic system was sampled and analyzed utilizing CA Title 22 Acute Fish Bioassay Screen Test for Hazardous Waste. The septic waste water sample was recorded as collected on a unique chain of custody. Nitrile gloves were utilized during the sample collection process. The sample was then shipped via Federal ExpreSSTM to McCampbell Analytical, lnc. located in Pittsburg, California for analysis. McCampbell Analytical, [lie. is accredited by the American Industrial Hygiene Association. This test is used to help determine whether or not a sample should be classified as a hazardous waste under state of California criteria, (these criteria are codified in Chapter 30 of Title 22 of the California Code of Regulations, Section 66696). Typically, the screen bioassay is conducted first, and then, if needed, followed by the definitive bioassay. Testing consists of mixing sub-samples with standardized laboratory water to produce specific test concentrations. The screen test uses three sample concentrations of 250, 500 and 750 mg/I, which bracket the 500 mg/I state criterion, and is conducted in duplicate. Ten fish are then added to each test tank and monitored for 96 howl. Water quality parameters, such as dissolved oxygen, pH, and temperature and mortalities are recorded daily. The final fish survival rate is used to determine whether or not the sample passes state criteria for non-hazardous waste, namely an LC50 greater than 500 mg/I (in other words, the concentration necessary to kill half of the exposed fish must be greater than 500 mg/1). If the sample narrowly fails the screening test, (40% or greater mortality in the 750 mg/1 concentration), a definitive test is recommended. The definitive test uses five concentrations bracketing the 500 mg/I criteria with the resulting data analyzed by computer to determine the LC50. Fathead minnows are typically used in this test, however, the golden shiner minnow or juvenile rainbow trout may be used, depending on regional preference. Results The waste water sample results showed a mortality rate of<40% at 750 mg/L. Therefore the waste water sampled fi-om within the septic system holding tanks is considered to be non-hazardous and can be disposed of as such (see laboratory documentation in Attachment A). Regards, AIA Maw Mike Ostlund Dr. Tianbao Bai, Pli D, CIH Project Manager Certified Industrial l lygenist 1900 MCHCNRY AVE., STE 201, GSCALON, CA 953200209.847.3800of FAX 209.847.3830 2