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W-Za <br />- +� <br />d � <br />Labos"ator'es Inc. �lFEB G 9 2011 <br />Environmental Telling Laboratory Since 1949 <br />Subcontract Report for 1632908 PDF File Name: wo_1632908_sub_all.pdf Page 2 of ENV(QNNI-AL �tC.:i H <br />�� <br />E)SPAH i rvi,Wt <br />FATHEAD MINNOW HAZARDOUS WASTE <br />SCREEN BIOASSAY "r Aquatic <br />Testing <br />Lab No.: A l b /1p9ol - o o l <br />Laboratories <br />Client/ID: 9 c- I d) t '1078 <br />TEST SUMMARY <br />Species: Pimephales promelas. <br />Fish weight (gm): av: 6:i. 3 ; min: O• Y) ; max: Q r S . <br />Reference Toxicant: SDS conducted per batch. <br />Test chamber volume: 10 liters. <br />Temperature: 20 +/- 2°C. <br />Aeration: none, unless D.O. drops below 5.0 mg/l. <br />Number of replicates: 2. <br />Dilution water: Soft reconstituted water (40-48 mg/l CaCO;). <br />TFCT BATA <br />Source: Thomas Fish. <br />Regulations: CCR Title 22. <br />Test Protocol: California F&G/DHS 1988. <br />Endpoints: Survival at 96 hrs. <br />Test type: Static. <br />Feeding: None. <br />Number offish per chamber: 10. <br />Photoperiod: 16/8 hrs light/dark. <br />Total Number Dead <br />INITIAL <br />11 <br />24 Hr 11 <br />48 Hr <br />11 <br />72 Hr <br />96 Hr <br />Date/Time: <br />(OL -16 <br />D <br />12.1-160)0 <br />12-2-1 <br />l0 <br />12-3-16 <br />1070 <br />12-14-16 to)D <br />Analyst: <br />I <br />5� <br />[ <br />"i, <br />:A <br />/ <br />°C I DO <br />pH <br />°C I DO I pH I d D <br />°C I DO <br />I pH I <br />p D <br />°C I <br />DO <br />I pH I <br /># D <br />°C I DO pH <br />k D <br />10- $, <br />S•o <br />, o l• ?-I D <br />2e, 0 4, <br />s• O <br />to. <br />2 •1 <br />4.0 <br />20 t Q. <br />.t' <br />L. . 3 ) G <br />70.1 e. <br />g, <br />O <br />�. <br />St•,r <br />9,1 <br />1..., g. d k, 0 <br />IMB <br />I9.Y 8•I <br />$.0 <br />1e.2 t,4 %9 C) <br />1a 2 <br />g,o <br />O <br />!0 <br />4.o <br />() <br />.,I 4.� 8.0 <br />6 <br />9 <br />6 7% <br />20, <br />v.V <br />O <br />,o <br />•o .S 8.0 <br />0 <br />l y Q,& <br />) <br />1, tc.t 9 d <br />)•Y <br />Ze.( <br />t• I <br />g.o <br />0 <br />0, &•Y .t <br />d <br />1 . S d.) <br />)- y <br />1.. ( St 2 7.4 <br />io 3 <br />7- <br />�. i <br />Q 2 <br />Y. O <br />b <br />o. l 8. 7.1 10 <br />Comments: <br />Extraction method: Mechanical shaking <br />None (aqueous solution) <br />Dissolved Oxygen (DO) readings in mg/l O,. Test Aerated: <br />& i No <br />Total Number Dead <br />CONTROL <br />HIGH CONCENTRATION <br />400 mg/l <br />Alkalinity Hardness <br />Alkalinity Hardness <br />Initial <br />) 2 mg/I CaCO, tl f mgA CaCO, <br />3 mgA CaCO,mgfl CaCO, <br />Final <br />mg/l CaCO, mgl Caco, <br />mg/lCaCO,P'(mgfiCaCO, <br />Total Number Dead <br />Control <br />U /20 <br />400 mg/l <br />Q /20 <br />750 m I <br />d/20 <br />RESULTS <br />the checked ✓ result applies based on fish survival rates of this test; NA - notapplicable) <br />PASSED <br />LC50 > 750 mg/I (<40% dead in 750 mg/I cont.) <br />NA <br />FAILED <br />240% dead in 750 mg/I (close to passing- definitive test recommended) <br />n/ -i <br />FAILED <br />LC50 < 400 mg/l (>60% dead in 400 mg/I cone.) <br />The results to this report apply to the samples analyzed ,n accordance wish the chain of custody document. This analytical report must be reproduced to its entirety. <br />All results listed in this report are for the exclusive use of the submitting party BC Labmatones, Inc assumes no responsibility for report alteration, separation, detachment or third parry interpretation <br />4100 Atlas Court Bakersfield, CA 93308 (661) 327-4911 FAX (661) 327-1918 www.bclabs.com Page 40 of 43 <br />