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Laboratories Inc. I� <br />Environmental Testing Laboratory Since 1949 <br />R -a' EE C�-IVE® <br />FEB Q 9 2017 <br />Subcontract Report for 1632908 PDF File Name: wo_1632908_sub_all.pdf Page 3 of 4 <br />Dic—PARTl IVEEN i <br />FATHEAD MINNOW HAZARDOUS WASTE <br />SCREEN BIOASSAY "r� Aquatic <br />Testing <br />QC Laboratories <br />Lab No.: r4/ 6 Il 14o (—oo 2 <br />Client/ID: 0 L 19 31 4 o 4 —0 3 A <br />TEST SUMMARY <br />Species: Pfinephales promelas. <br />Fish weight (gm): av: 6 - 3 ; min: 0. N max: 0• S'8 . <br />Reference Toxicant: SDS conducted per batch. <br />Test chamber volume: 10 liters. <br />Temperature: 20 +/- 2nC. <br />Aeration: none, unless D.O. drops below 5.0 mg/I. <br />Number of replicates: 2. <br />Dilution water: Soft reconstituted water (40-48 mg/1 CaCO,). <br />Date/Time: <br />Analyst: <br />TEST DATA <br />Source: Thomas Fish. <br />Regulations: CCR Title 22. <br />Test Protocol: California F&G/DHS 1988. <br />Endpoints: Survival at 96 hrs. <br />Test type: Static. <br />Feeding: None. <br />Number of fish per chamber: 10. <br />Photoperiod: 16/8 hrs light/dark. <br />Control A <br />4-1 F. t ff•o <br />0 8- <br />D O <br />L <br />l Alkalinity Hardness <br />Control B <br />lbi Q. .V <br />q <br />a• 1 8. 3% I <br />b <br />?iJ• I d 7 Q 0 <br />8• S ti, ( �j <br />D f• 6 fi .p U <br />400mgIA <br />7 S•i g•0 <br />til 4.y )qQ <br />2a. 8.4 7 6 <br />20.2 4.0 0 <br />11 0 <br />8. S.o <br />400 mg/I B!_ <br />Q E 8 •0 <br />. 3 7 it O <br />7.1f.1 <br />9-0 0 <br />2 2. s j. 4 0 <br />750 mg/I A <br />7 91 g. 0 <br />) <br />7. O <br />, t 77. OO <br />7 D <br />m B <br />7 <br />. 4 O <br />$7 <br />1.1 <br />c , l <br />GF7750 <br />Comments: Extraction method: Mechanical shaking t/ . <br />None (aqueous solution) �✓�. <br />Dissolved Oxygen (DO) readings in mg/l O,. Test Aerated: YID / No <br />Total Number Dead <br />CONTROL <br />HIGH CONCENTRATION <br />400 mg/I <br />Alkalinity Hardness <br />l Alkalinity Hardness <br />Initial <br />N 2 moCaco, lr f mglt CaCO, <br />3 mg/I Caco, mg/I Caco, <br />Final <br />3 mgA CaCO, yIj mgil CaCO, <br />3 z mg/I Caco, mgJ CaCO, <br />Total Number Dead <br />Control <br />0 /20 <br />400 mg/I <br />/20 <br />750 m I <br />6 /20 <br />RESULTS <br />the checked ✓ result applies based on fish survival rates of this test; NA - notapplicable) <br />PASSED <br />LC50 > 750 mg/1 (<40% dead in 750 mg/I cone.) <br />FAILED <br />X40% dead in 750 mg/1 (close to passing - definitive test recommended) <br />FAILED <br />LC50 < 400 mg/1 (>60% dead in 400 mg/I cone.) <br />The results in this report apply to the samples analyzed in accordance woh the chain of custody docunreni. This analytical report must he reproduced to its entirety. <br />All results listed in this repon are for the exclusive use of the s ibmntmg party BC Lab—i—es. Inc assumes no responsib,hty for report aheranon, separation, detachment or third party mterprew- <br />4100 Atlas Court Bakersfield, CA 93308 (661) 327-4911 FAX (661) 327-1918 www. bclabs corn Page 41 of 43 <br />