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Pacific EcoRiskEnvironmental Consulting &Testing <br /> 1. INTRODUCTION <br /> Under contract to GeoAnalytical Laboratories, Inc. (GeoAnalytical), Pacific EcoRisk (PER) <br /> conducted a definitive Title 22 "Hazardous Waste" fish toxicity evaluation of a solid waste <br /> sample, designated "R200875 IA". This toxicity test consisted of a 96-hr static bioassay with <br /> juvenile fathead minnows (Pimephales promelas). This report describes the performance and <br /> results of this test. <br /> 2. ACUTE TOXICITY TEST PROCEDURES <br /> The methods used in conducting this test followed the guidelines established by the California <br /> Dept. of Fish & Game manual "Static Acute Bioassay Procedures for Hazardous Waste Samples" <br /> (Polisini and Miller 1988), with additional guidelines as provided by the California Dept. of <br /> Health Services (Janna White, personal communication). <br /> 2.1 Receipt and Handling of the Waste Sample <br /> A solid waste sample, designated "R200875 IA", was collected by GeoAnalytical staff on <br /> August 18, 2005, and was delivered August 30,2005 to the PER testing laboratory in Martinez, <br /> where it was placed under refrigeration at 4°C until used to prepare the test solutions. The chain- <br /> of-custody record for the collection and delivery of this waste sample is provided in Appendix A. <br /> 2.2 Test Organisms <br /> The Pimephales promelas used in this test were obtained from a commercial supplier (Thomas <br /> Fish Company,Anderson,CA). These fish were maintained at 20'C in aerated aquaria <br /> containing EPA synthetic soft water prior to their use in this test. During this pre-test period, the <br /> fish were fed brine shrimp nauplii ad libitum. <br /> 2.3 Preparation of the Test Media <br /> Sub-samples of the waste were mixed with the Control/dilution water for 6 hrs, using a wrist- <br /> action shaker. The Control/dilution water, designated as "Lab Control," consisted of synthetic <br /> U.S.EPA "soft" water (prepared by adding reagent grade salts to reverse-osmosis de-ionized <br /> water). After mixing, these preliminary solutions were used to prepare test solutions at sample <br /> concentrations of 250,400,500,600, and 750 mg/L. <br /> Page I <br />