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Pacific EcoRisk is • • <br /> f Environmental Consulting &Testing <br /> 2.4 Acute Toxicity Testing with Fathead Minnows <br /> This hazardous waste evaluation was run at sample concentrations of 250,400, 500, 600, and <br /> 750 mg/L, with a Lab Control consisting of the same synthetic EPA "soft" water. Water quality <br /> characteristics (pH and dissolved oxygen [D.O.1) were determined for the test solution in each <br /> test replicate prior to initiation of this test.There were 2 replicates at each of the treatments, each <br /> replicate consisting of 9.2 L of media in a 10-L plastic container.The test was initiated with the <br /> random allocation of 10juvenile fathead minnows into each replicate container. These <br /> containers were placed in a temperature-controlled water bath at 20°C, under fluorescent lighting <br /> on a 16L:8D photoperiod. <br /> Each day, each replicate container was examined,and the number of live fish in each was <br /> recorded. Routine water quality characteristics (temp, pH, and D.O.) of the treatment waters <br /> were measured and recorded for each replicate container each day. <br /> After approximately 96 hrs, the test was terminated and the number of live fish in each replicate <br /> container was determined.The fish in a randomly selected Lab Control treatment replicate were <br /> then euthanized and measured for total length (to the nearest 0.1 mm) and weighed (to the <br /> nearest 0.01 g).The resulting survival data for the waste treatments were evaluated to determine <br /> the LC50 using the CETIS®statistical software (Version 1.025a,TidePool Scientific, <br /> McKinleyville, CA). <br /> Page 2 <br />