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Pacific EcoRisk Environmental Consulting&Testing <br /> 1. INTRODUCTION <br /> Under contract to GeoAnalytical Laboratories, Inc. (GeoAnalytical), Pacific EcoRisk(PER) <br /> conducted a screening Title 22"Hazardous Waste"fish toxicity evaluation of a waste sample, <br /> designated"Fly Ash A".This toxicity test consisted of a 96-hr static bioassay with juvenile <br /> fathead minnows(Pimephales promelas). This report describes the performance and results of <br /> this test. <br /> 2. ACUTE TOXICITY TEST PROCEDURES <br /> The methods used in conducting this test followed the guidelines established by the California <br /> Dept. of Fish&Game manual "Static Acute Bioassay Procedures for Hazardous Waste Samples" <br /> (Polisini and Miller 1988), with additional guidelines as provided by the California Dept. of <br /> Health Services (Janna White, personal communication). <br /> 2.1 Receipt and HandUng of the Waste Sample <br /> A waste sample,designated"Fly Ash A", was delivered on March 1,2007,to the PER testing <br /> laboratory in Martinez, where it was placed under refrigeration at 4°C until being used to prepare <br /> the test solutions. The chain-of-custody record for the collection and delivery of this waste <br /> sample is provided in Appendix A. <br /> 2.2 Test Organisms <br /> The Pimephales promelas used in this test were obtained from a commercial supplier(Thomas <br /> Fish Company,Anderson,CA).These fish were maintained at 20°C in aerated aquaria <br /> containing EPA synthetic soft water prior to their use in this test. During this pre-test period,the <br /> fish were fed brine shrimp nauplii ad libitum. <br /> 2.3 Preparation of the Test Media <br /> Sub-samples of the waste were mixed with the Lab Control/dilution water for 6 hrs, using a <br /> rotary mixer.The Lab Control/dilution water consisted of synthetic US EPA"soft" water <br /> (prepared by adding reagent grade salts to reverse-osmosis de-ionized water). After mixing,these <br /> preliminary solutions were used to prepare test solutions at sample concentrations of 250, 500, <br /> and 750 mg/L. <br /> Note- Due to elevated pH in all test treatment test solutions(i.e.,>8.5 pH units),the pH was <br /> adjusted to 8.5 f 0.1 with reagent grade HCI;a pH-adjustment Control treatment was initiated <br /> concurrently to evaluate any potential effects of the HCl addition. <br /> Page I <br />