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Pacific EcoRisk Environmental Consulting&Testing <br /> 2.4 Acute Toxicity Testing with Fathead Minnows <br /> This hazardous waste evaluation was run at sample concentrations of 250,500,and 750 mg/L, <br /> with a Lab Control consisting of the same synthetic EPA "soft" water. Water quality <br /> characteristics(pH and dissolved oxygen [D.O.])were determined for the test solution in each <br /> test replicate prior to initiation of this test. There were 2 replicates at each test treatment, each <br /> replicate consisting of 4.2 L of media in a 10-L plastic container.The test was initiated with the <br /> random allocation of 10 juvenile fathead minnows into each replicate container. These <br /> containers were placed in a temperature-controlled water bath at 20°C, under fluorescent lighting <br /> on a 16L:8D photoperiod. <br /> Each day, each replicate container was examined, and the number of live fish in each was <br /> recorded. Routine water quality characteristics(temperature,pH,and D.O.)of the treatment <br /> waters were measured and recorded for each replicate container each day. <br /> After 96(±2)hrs,the test was terminated and the number of live fish in each replicate container <br /> was determined.The fish in a randomly selected Lab Control treatment replicate were then <br /> euthanized and measured for total length(to the nearest 0.1 mm)and weighed (to the nearest <br /> 0.01 g).The resulting survival data for the waste treatments were evaluated to determine the <br /> LCSo using the CETIS®statistical software(Version 1.1.2revL,TidePool Scientific, <br /> McKinleyville, CA). <br /> Page 2 <br />