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'�..._.—F -0'3082- -: <br /> ENE <br /> rF' YEN. <br /> ;�iri� a• <br /> '��nL�k '2 a `S Y �`i: trv'x F ��:.�R� <br /> + a <br /> ���9'p}��i,asa <br /> ���.v!r''M.�i%�5 u"rs:#'��}L#' '��"`�'6 �'���y<. 'Sj .r •�.'F't �X9'3 _ 1' f�' � y .7rt:h�'+ a Fre.Sr: <br /> 2 <br /> y* <br /> 2 <br /> 1 <br /> Du Pont HLR 399-87 <br /> Title of Report <br /> t: NUTRIENT OPTIMIZATION STUDIES OF LODI SAMPLES <br /> TO DETERMINE BIODEGRADATION POTENTIAL <br /> Summar <br /> Two soil slurries from different mites at Lodi, California avers teoted to <br /> predict the optimalmicrobial degradation of gasoline components, upon <br /> nutrient addition. Nutrient optimization atudiere employed radiolebeled <br /> Xylene to determine the fate of this relatively recalcitrant chemical in tha <br /> t prasence of 18 different combinations and concentrations of nutrients. <br /> ` (phosphorus source, nitrogen source, and trace acetals). The nutrient <br /> } treatment which provided the highest level of mineralized xylene (expressed <br /> s' vas evolved radiolabeled CO.,) was different for the two moil samplos. The <br /> nutrient treatment containing 40 ppm phosphate (P) and 100.ppm ammonium <br /> chloride (N) ranked high (greatest mingrslization) for both nail slurries, <br /> followed by: 40 ppm P and 50 ppm N (low nutrient treatment); and, 80 ppm P <br /> and 100 ppm N (medium nutrient treatment). <br /> The low and medium nutrient combinations 'in the absence of trace metals <br /> x were chosen for ® 900-fold scale-up teat. Microbial activity was enhanced <br /> (as determined by adenosine triphosphate analysis) for both of these <br /> treatments after two weeks of incubation; and the medium nutrient treatment <br /> Increased the microbial activity with a concomitant lose of ben:xena, toluenep <br /> and xylene. These data suggest that the medium nutrient combination f <br /> (80 ppm P'and 900 ppm N) would be appropriate for in situ bioreclamation. . <br /> Reported bya <br /> • sig A. a cher Y r <br /> Immunochemist <br /> alb s� essLzka a!o ay yr <br /> - <br /> Technician <br /> A <br /> Approved by: 8 <br /> } .. rank hil pe No/Day/Yr <br /> Section Supervisor . <br /> Environmental Toxicology <br /> and Information Services r <br /> LAB/WEB/FXP/egg/NIRP 8.9: <br /> _ ; <br /> R <br />