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AW t Du Pont HLR 599-87 release of carbon dioxide (this timr. was ehartensd to 30 min* for the first soil slurry, M-18779). The plastic buckets containing the filter paper were clipped into 10 mL of scintillation fluid (Aquasol) end were counted in a TH Analytic Mark III (6882) liquid scintillation system to obtain disintegrations per minute (DPH) for each bottle. The percent of total labeled xylene mineralized was determined by dividing the corrected DPH (minus the formaldehyde control) from each test bottle by the total DPM10 (379,804) added and eultiplying.by 100. A small separate experiment was performed to cheek whether another CO trapping chemical mould provide better results at later time periods for he groundwater eaample. The above procedure was followed except that 0.2 mi. of a 5 6N solution of NON was added to the filter in place of PEA. Treatments 5 and 14 (Table 2) were tested in groundwater (aa received) using this method. r. Scale-up Study Using Adenoaine Triphosphate and Chemical Analyses Nutrient combinations and concentrations were chosen from the above E studies and repeated on a scaled-up basis (100X). Nutrient treatments 5 and 9 (Table 2) were selected for the scale-up studies. ' Nutrients were added to two liter media battles. A 25: soil slurry (M-18774) was prepared for a total volume of 1666 mL. A mixture of benzene, toluene, and xylene (in equal { volumes) was added (final concentration of 0.21 ppm) and a Teflon® lined cap with three sample ports was tightly fitted to the bottle. This come mixture of chemicals was also added to the battles on day 9. Subsemples were collected on days 0, 2, 5, 7, 9, 12, and 14 of the water phase and the soil azurry by attaching s syringe to the sample port and removing small aliquots. Equal amounts of the.soil slurry and an ATP releasing agent were vortexed together and the particulate metier brought out of solution by centrifugation. The supernatant was evaluated for ATP. The water samples mere directly tested without additional manipulation. Fluctuations in total f biomass were determined by measuring mdenceine triphosphate (ATP) levels. E All living cella contain ATP while ATP from dead and dying cella in rapidly hydrolyzed. Therefore, measurement of ATP is a good indicator of the presence of microorganisms. The ATP eras measured using the LINAC Biocounter® and a standard curve wga made with a commercial source of ATP. Twenty-five williliter sampled were collected at the some time periods as above and placed in mmberglass vials with Teflon$ lined caps and no air apace. These samples were stored in the refrigerator until time of analysis st the end of the experiment by Artesian Laboratories, Inc. The analyses were performed by extracting the soil slurries with methanol. Ij 4