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• MEMCRANDUM CALIFORNIA REGIONAL WATER QUALITY CONTROL BOARD • CENTRAL VALLEY REGION 3443 Routier Road, Suite A % Phone: (916) 255-3000 '. Sacramento, CA 95827-3098 CALNET: 8-494-3000 TO: Gordon L. Boggsr` FROM: Elizabeth A. Thayer UGT Program Coordinator Associate Engineer DATE: 3 June 1994 SIGNATURE: �iLi� ,wZ SUBJECT: BIOREMEDIATION PILOT STUDY, GEWEKE, 16 SOUTH CHEROKEE LANE, LODI, SAN JOAQUIN COUNTY On 2 February 1994, monitoring well MW4 and vapor wells VWl, VW4, VW5, and VW6 were inoculated with bacteria as part of a Bioremediation Pilot Study. On 4 February 1994, I met with Bob Marty and Vic Cherven, of Geological Audit Services (GeoAudit), Geweke's consultants, to assist them in setting up a monitoring program for the Pilot Study. Mr. Cherven has since left GeoAudit. My memorandum, dated I March 1994, describing the monitoring program, is in the file. The wells selected for inoculation of bacteria either did not extend to ground water or packers were used during inoculation to prevent the bacteria from reaching ground water. The Pilot Study was conducted during the months of February and March 1994. Several different monitoring methods were used to monitor for bacteria in soil and ground water and for oxygen, carbon dioxide, and hydrocarbons in the soil and in ground water.The monitoring methods were chosen based on the theory that the bacteria would move through the soil and could be detected in soil, the bacteria may also move to the ground water and could be detected in ground water, and the byproducts of bacterial metabolism could be detected in air and ground water samples. The Pilot Study was completed at the end of March. A report describing the results of the February monitoring were submitted in a report entitled "Monthly Monitoring Report", dated 11 March 1994. A compilation of the data from both the February and March monitoring was submitted in tabular form by FAX on I1 and 12 May 1994. I reviewed the 11 March report, the tables sent by FAX, and the most recent "Quarterly Report: March 1994". Copies of the pertinent tables are attached to this memo and the following comments are organized in the same order as the tables. Figure 1 shows the Pilot Study area, all the wells involved in the Pilot Study, the inoculated wells, and the wells _ attached to the air blower during the Pilot Study. FAXED TABLES - 11 AND 12 MAY 1994 TABLE 1: ANALYTICAL RESULTS OF WATER SAMPLES, Bacteria - Number & Species Even though the bacteria were inoculated only into the soil, the bacteria might have migrated to ground water. Therefore, several ground water monitoring programs were set up depending on whether the bacteria were detected in ground water during February's sampling. Ground Water Monitoring Program A was the initial monitoring program for analysis of bacteria in water. The inoculated bacteria were not detected in ground water and none of the other analyses indicated that the bacteria reached ground water during February. Therefore, Ground Water Monitoring Program A was used throughout the Pilot Test. (Program A is described in the 1 March 1994 memo.)