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.s BIO-RE B, I19C. 'S AUGME MD 113 SITU SUBSURFACE BIOMMDIATIM PRCCES% A. Description of Technology. Bio-Rem, Inc. 's Augmented In Situ Subsurface Bioremediation Process.M incorporates four (4) steps in the subsurface bioremediation of hydrocarbon contasination in soil and water. This process occurs totally underground. It utilizes Bio-Rem, Inc. 's Product °H-102, a proprietary blend of microaerophilic bacteria and micronutrients. It does not require additional nutrient loading after placement, nor does it require the addition of oxygen or oxygen producing compounds, e.g. hydrogen peroxide. As this process occurs underground and the degradation products of the process produce only naturally occurring elements, this process complies fully with Title I of the Clean Air Act Amendments. 1. STEP ONE. The first step in this process is a detailed and accurate definition and characterization of the contaminate plume. The definition and characterization of a site gives the geology, hydrogeology and gradient of the site. It also provides the dimensions of the contaminate plume. The accurate definition and characterization of the contaminate plume is necessary for Step Two of this process. 2. STEP TWO. The second step of the process is to determine the Application Methodology. Application of the proprietary bacterial cultures can be accomplished in a variety of methods. If the soil composition is permeable, only borings and wells need to be drilled into the contaminate plume. If the soil is mixed or is tight clay, the boring/wells, as well as trenching and lancing may be required. The selection of the appropriate Application Hethodology is site specific. 3. STEP TERES. The third step is the initialization and propagation (growth) of the proprietary bacterial cultures. The product that is initialized and groan (Sio-Rem H-10) contains a selected range of microorganisms and micronutrients. The microorganisms are called Omicrooerophilic° because they utilize the available oxygen contained in subsurface soil and groundwater. A natural water source is used to initialize and grow the cultures on site. This "Hatch Solution° is then placed into the contaminate plume via the Application Hethodology previously described. The amount of culture and batch solution placed into the contaminate plume is determined by a proprietary formula. 4. STEP FUDR. The fourth step is that of monitoring and reporting. After the proprietary cultures are placed into the contaminate plume, readings are taken at periodic intervals. This is accomplished by using a Photoionization Detector (PID). PID readings are taken from the monitor hells/borings placed in and around the contaminate plume. When the readings fall below required °clean-up" leveler, soil and/or water samples are taken from the site and sent for formal analysis by a certified, independent laboratory. These results confirm that the site is below clean-up levels and is thus QHot Actionable".