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The Availability of Energy Sources for Baeterl1 Metabolism/Growth. <br /> The oil-consuming bacteria do so by producing extracellular digestive enzymes which attack the petroleum <br /> molecule, break it down into smaller pieces, and render it more easily metabolized. The process of metabolism <br /> releases energy which in turn makes the biodegradation process move more swiftly. If nutrients and metabolic <br /> enzymes are added to the contaminated soil, the bacteria can more swiftly accelerate their digestive activities. <br /> The faster the metabolic process develops, the more energy is released to further speed up the reaction; a <br /> positive-feedback closed-loop system develops, with an increase in the rate of petroleum cleanup by a factor of <br /> some thirty times or more. Some petroleum product not readily degradable by bacteria is transformed by co- <br /> metabolism into more readily degradable forms. <br /> By providing a variety of carbon-sources for bacterial metabolism. FyreZyme enables the existing bacteria, <br /> both oil-degrading and non-oil-degrading to increase their metabolic activity, creating a high energy system far <br /> in excess of that prior to such treatment. By providing a rich supply of extracellular enzymes, FyreZyme <br /> further accelerates the petroleum breakdown and favors the growth of oil-consuming bacteria. <br /> The Availability of Oxygen in Sufficient Quantity to Support Metabolism <br /> \%--'As the vast majority of petroleum breakdown is performed by bacteria that utilize oxygen in their metabolic <br /> processes, it is necessary to ensure that oxygen is available in suitable quantities. This is provided in the <br /> upper soil layers (2 ft.) by mixing FyreZyme with surface water, oxygenated aquifer water, and or a <br /> combination of water and atmospheric air which is naturally rich in oxygen. <br /> If the contamination is deep in the soil and the conditions are anaerobic, a combination of FyreZyme, <br /> oxygenated water, air, and other sources of oxygen can be injected. Hydrogen peroxide starting at 0.05% can <br /> be used for oxygenation without adversely affecting the bacterial colonies. Ozone has special toxic qualities <br /> which make its' use more difficult. Movement of the nutrient and oxygen through the deeper layers of the <br /> vadose and beyond can be accomplished by strategically sited injection/recovey wells. Addition of FyreZyme <br /> to soil venting operations has proven extremely beneficial. <br /> It can be seen that treatment of excavated material is the most efficient for aerobic bioremediation, but similar <br /> results can be obtained by in-situ application of the FyreZyme used in conjunction with air injection, <br /> sparging, venting, and a wide variety of other technologies suitable to provide oxygen in deeper soil layers. <br /> L <br />