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1 <br /> A. Keith Kaufman, M.S. <br /> Consultant in Environmental Biotechnology <br /> Serving Industry,Government.Academia and the Legal Profession <br /> ' June 18, 1999 <br /> ' TO: Mr. Bill Little (Advanced GeoEnvironmental, Inc,) <br /> FROM: A. Keith Kaufman V <br /> Subject: Results of Physicochemical and Microbiological Analyses of Two (2) Soil <br /> Samples (Olympian Oil) <br /> 1 <br /> Listed below are the results from the referenced analyses performed on two (2) soil samples <br /> ' collected on 5127199 and received on 612199. After appropriate dilution, samples were plated <br /> onto TSA and MSA supplemented with 1500 ppm of a gasoline/diesel/motor oil mix as sole <br /> carbon substrates. Media were prepared at ambient pH. Cultures were incubated under <br /> aerobic conditions at room temperature for six (6) days prior to enumeration. Physicochemical <br /> analyses were performed in accordance with previously described protocol. <br /> RESULI� <br /> Parameter__ . Bl-25 <br /> pH 8.6 8.4 <br /> Nitrate-N (ppm) 7.0 6.0 <br /> ' Nitrate (ppm) 30.8 26.4 <br /> Ammonia-N (ppm) ND ND <br /> Ammonium (ppm) ND ND <br /> ' Ortho-P (ppm) 12.6 11 .7 <br /> Potassium (ppm) 128 62 <br /> ' % Moisture 5.8 13.9 <br /> ' Gen. Micr. Enum. <br /> (CFU X 1021gr) 8.0 10.0 <br /> Sel Micr. Enum. <br /> (CFU X 1021gr) ND ND <br /> ' % Biodegraders --- -- <br /> ' ND; Not detected at limit of 0.1 ppm/10,2 dilution <br /> CFU; Colony-forming Units (viable cells) <br /> United States Office Asia-Pacific Office <br /> 6245 Bristol Parkway 11249 2nd Floor,t#I62 Tung-An Street,Ta-An District <br /> ' Culver City,CA 90230 Taipei,Taiwan R.O.C. <br /> (310)822-9385 (310)577-9364[FAX] 886-2-378-3751 886.2-378-3752[FAX] <br />