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Advanced GeoEnvironmental, Inc. <br /> ' Olympian Oil <br /> June 18, 1999 <br /> ' Page 2 <br /> OQMMEI�II <br /> ' The samples submitted for analyses revealed very low viable recoveries for heterotrophic and <br /> selective microorganisms at the dilutions tested. Neither sample yielded any selective <br /> microorganism growth. In general, bionutrient conditions are within the range of support for <br /> t microbial growth and metabolism, except for low levels of ammonium. Although low levels <br /> of ammonium may be expected to result in reduced viable cell recovery, the levels of <br /> suppressed growth found in these samples would not be expected. These results suggest the <br /> ' possibility that some level of biotoxicity exists at the site, and efforts should be considered in <br /> order to further evaluate this possibility. Such biotoxicity can be due to excessive levels of <br /> metals, salt, or other, as yet unidentified material. Alternatively, carbon substrate (either <br /> ' contaminant-based or competitive forms) may be present at levels which are insufficient to <br /> support microbial growth and would thus help to explain the low growth values obtained. It <br /> is possible that both conditions may coexist. An evaluation of analytical chemistry associated <br /> with these samples (hydrocarbon levels 1 total carbon levels) would assist in reconciling these <br /> results. Based on the present results, it is unlikely that natural bioattenuation would result in <br /> significant levels of contaminant reduction. <br /> Should you have any questions concerning these results, please do not hesitate to contact me. <br /> 1 <br /> AKK:ch <br /> 1 <br /> ;t <br /> IA <br />