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Procedures Approximately 5 pounds of contaminated soil was collected from the specified location and shipped to BioJet Upon receipt of the samples at the lab, BioJet staff combined the brass tubes from the location to form one composite Once the sample was combined baseline physicochemistry (nutrient levels) and microbial enumerations were conducted After the initial baseline testing, liquid nutrients were added to the to achieve approximately 65 ppm of Nitrate (NO3) The sample was then loosely sealed and allowed to incubate at room temperature (average temperature 60-65 degrees Fahrenheit) for a period of 21 days The supplemented studies were analyzed at 4,7,14 and 21 days Each time samples were collected they were analyzed for the following parameters pH, Nitrate (NO3), Ammonium (NH4), Phosphorous (PO4), and Potassium (K), and microbial enumerations, which consisted of General and Selective Enumerations According to data supplied by AES the sample from VE-1 was sampled at a depth of 25 feet, Baseline samples showed that nutrient levels were in what would be considered acceptable ranges especially for Nitrate (see Exhibit 1 for complete results) Nitrate levels were reported at 27 ppm, however the microbial enumerations were relatively low with heterotrophic and selective counts of 8 and O1 respectively and only 13% degraders The low recovery of selective organisms could indicate that there is not enough contaminant to support large populations at this location Once the baseline analyses were concluded nutrients were added to the sample to reach approximate levels of 65 ppm nitrate as well as enhancing the remaining nutrient parameters (NHS, Po&K) The sample was then loosely sealed and left undisturbed at room temperature (60OF +/- 50) At 4 days, the sample was analyzed for both physicochemistry and microbial enumerations The results showed that nitrate levels had increased to 58 ppm, while microbial enumerations for general and selective increased to 12 4 and 2 1 respectfully and 16 94% degraders At 7 days, the results showed that nitrate levels had decreased to 50 ppm, while microbial enumerations for general and selective increased to 16 5 and 2 4 respectfully and 14 55% degraders At 14 days, the results showed that nitrate levels had decreased to 40 ppnn, while microbial enumerations for general and selective increased to 17 2 and 3 5 respectfully and 20 35% degraders At 21 days, the results showed that nitrate levels had increased to 42 ppm, while microbial enumerations for general and selective decreased to 16 9 and 3 2 respectfully and 18 93% degraders These results indicate that the introduction of the nutrients as a food source may have enhanced activity for the microbial populations The remaining physicochemistry parameters remained relatively the same, less then 15% change over the period of the study (see Exhibit 1), as would be expected since the uptake of nitrate is the easiest process for the microbes which will later utilize the other parameters when nitrate levels become limiting Over the course of the study nitrate levels dropped 28% from baseline, while the general population increased i 1 fold aald the selective counts increased 320 fold, resulting in an increase of 145 fold in overall degrader kinetics for the study