Laserfiche WebLink
Effects of 01yRen Augmentation <br /> This study's were performed over a 21-day period, and conducted to comparatively <br /> evaluate the effects of oxygen augmentation on microbial kinetics The studies involved <br /> samples from location VE-1 taken at a depth of 40 feet from the site This study was <br /> undertaken to determine if oxygen levels at the site may be a contributing factor in <br /> limiting microbial growth and biodegradation, and to determine what effect oxygen <br /> augmentation would have on microbial growth <br /> Procedures <br /> Approximately 5 pounds of contaminated soil was collected from the specified location <br /> and shipped to BioJet Upon receipt of the samples at the lab, BioJet staff combined the <br /> brass tubes for the location to form one composite Once the sample was combined <br /> baseline physicocheinistry (nutrient levels) and microbial enumerations were conducted <br /> After the initial baseline testing, oxygen enriched water was added to increase available <br /> oxygen to the microorganisms (mixing of the soil could have also increased oxygen <br /> levels) The sample was then loosely sealed and allowed to incubate at room temperature <br /> (average temperature 60-65 degrees Fahrenheit) for a period of 21 day The augmented <br /> study was analyzed at 4,7,14 and 21 days Each time samples were collectea they were <br /> analyzed for the following parameters pH, Nitrate (NO3), Ammonium (NH4), <br /> Phosphorous (PO4), and Potassium (K), and microbial enumerations, which consisted of <br /> General and Selective Enumerations <br /> According to data supplied by AES the sample from VE-1 was sampled at a depth of 40 <br /> feet, Baseline samples showed that nutrient levels were in what would be considered <br /> acceptable ranges especially for Nitrate (see Exhibit 1 for complete results) Nitrate <br /> levels were reported at 32 ppm, however the microbial enumerations were relatively low <br /> with heterotrophic and selective counts of 12 and 03 respectively and only 25% <br /> degraders The low recovery of selective organisms could indicate that there is not <br /> enough contaminant to support large populations at this location Once the baseline <br /> analyses were concluded oxygen was added to the sample The sample was then loosely <br /> sealed and left undisturbed at roori temperature (WF 1/- 50) <br /> At 4 days, the sample was analyzed for both physicochemistry and microbial <br /> enumerations The results showed that nitrate levels had increased to 34 ppm, while <br /> microbial enumerations for general and selective increased to 13 and 12 respectfully and <br /> 9 23% degraders At 7 days, the results showed that nitrate levels had decreased to 26 <br /> ppm, while microbial enumerations for general and selective increased to 17 and 2 1 <br /> respectfully and 12 35% degraders At 14 days, the results showed that nitrate levels had <br /> d to 212 1.. 1 i„ ] A.,,,r.�or ti^n3 fbr general and selective were 15 and <br /> . <br /> decreased LV L �Jt1111, WJ-�-lie uuvi�J unit �.iiuw.•+ia <br /> 24 respectfully and 16% degraders At 21 days, the results showed that nitrate levels <br /> were 23 ppm, while microbial enumerations for general and selective were 12 and 2 3 <br /> respectfully and 19 17% degraders These results indicate that the introduction of oxygen <br /> inay have enhanced activity for the microbial populations <br />