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5. The c t sterilized load is to be left in the chambntil the incubation test <br />resultinfirmed to be negative. 4 <br />a. A sign will be placed on the door of the chamber indicating <br />"TEST IN PROGRESS, DO NOT OPERATE". <br />F. Incubation and Readout Procedure using the 3 Tm AttestTm Auto -Reader <br />1. While wearing PPE, the EVS Aide opens the 3M Biological Indicator Test Pack <br />and removes the Biological Indicator Tube. <br />2. The Engineer then closes the biological cap by pressing down. <br />3. The Engineer crushes the glass ampule in the designated crushing -well built into <br />the indicator. <br />4. The Engineer holds the biological indicator by the cap and taps the bottom of the <br />vial on countertop until growth media wets the spore strip at the bottom of the vial. <br />5. The Engineer places the biological indicator in the 3MTM AttestTm Auto -Reader <br />well. <br />a. Do not remove the biological indicator once it is placed in the <br />well. Otherwise, the test will be invalid. <br />6. Close the cover and wait for results at least 3 hours. <br />G. How to Read the Results <br />1. The Engineer (or designee) reads the results after 3 hours of incubation. <br />2. Negative Results (green light -): Indicates an acceptable sterilization process. <br />a. The Control Tube will always be Red (positive), and the sterilized <br />tube should always be Green (negative). <br />3. Positive results (red light +): Indicates an unacceptable sterilization process. <br />a. Control tube will be Red (positive), and the sterilized tube will be <br />Red (positive). <br />b. Control tube will be Green (negative), and the sterilized tube will <br />be Red (positive) or Green (negative). <br />4. In the event of a positive test result, retest the Sani-Pak and do not use the Sank <br />Pak for processing and sterilizing loads until three consecutive indicator <br />results are negative. <br />5. After not achieving three consecutive negative indicator results, the Engineering <br />Department will schedule repairs either internal or through the Sani-Pak Vendor. <br />a. This load and all untreated infectious waste will be placed into <br />Biohazard Waste Containers for off-site waste treatment through <br />Stericycle, until the Sani-Pak is operational again. <br />b. Once repairs have been completed, retest the Sani-Pak and do <br />not place back into service until three consecutive negative <br />indicator results have been achieved. <br />6. After incubation, if the control tube is also green, the Environmental Services <br />Department will contact the manufacturer to report the results, and will order a <br />new batch of biological indicators. <br />Spore Testing/EC.SA.081 Page 3 of <br />