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C. Gloves. Double gloves are to be worn by those who handle or <br />dissect unfixed specimens. In addition, when large or bloody spe- <br />cimens are handled, facial barrier precautions are to be followed. <br />3. Type of Specimens (The exterior of all specimen containers shall be <br />considered contaminated.) <br />a. Frozen Sections - Gloves shall be worn during frozen sections. <br />b. Smears - Imprints, cytological smears, bone marrow preparations, <br />and body fluid smears are considered contaminated until fixed or <br />stained and covered. <br />C. Routine Specimens - Routine specimens are to be fixed at the point <br />of collection. Adequate fixation (volume of fixative) will be <br />verified upon delivery to.pathology or at the time of gross des- <br />cription. <br />d. Body Fluids - Body fluids used to prepare smears or blocks are <br />extremely hazardous. While handling large quantities of these <br />fluids, protective clothing, gown/apron, double gloves and facial <br />protection is required. In addition, if the risk of droplet dis- <br />persal is high, the use of the biological safety cabinet is <br />required. If available, safety centrifuge cups are to be used <br />during these procedures. <br />e. Other - Teeth, calculi, implants and foreign bodies shall be <br />handled as tissues. Paraffin blocks and covered slides shall not <br />be considered infectious. <br />4. Decontamination <br />a. Any spills of blood or body fluids shall be cleaned immediately <br />with tuberculocidal disinfectant or diluted bleach (1:10 household <br />bleach). <br />b. Large spills of formalin (>500 ml) shall be cleaned immediately <br />with the appropriate spill cleanup kit. <br />C. All work surfaces and areas adjacent to the work area shall be <br />cleaned, decontaminated and flushed with water. <br />d. All instruments shall be decontaminated and rinsed with water. <br />Alternatively, disposable instruments and "sharps" shall be dis- <br />posed of in a puncture -resistant container. <br />e. All contaminated disposable clothing and supplies shall be handled <br />as infectious waste. Reusable clothing shall be handled as infec- <br />tious linen. <br />f. The cryostat- shall be decontaminated with 100% alcohol after each <br />day of use. <br />g. The cryostat shall be defrosted, claned of accumulated trimmings <br />and sections, and decontaminated with a tuberculocidal disinfec- <br />tant once each month and after tissue with M. tuberculosis is cut. <br />Gloves and facial protection shall be worn during this cleaning <br />procedure and extreme care taken when handling the microtome kni- <br />ves. <br />5. Tissue Waste Disposal <br />a. Fixed anatomical/pathological specimens will be stored in -the pri- <br />wary specimen container within the Histopathology laboratory until <br />the case has been examined and reported out by,the Pathologist. - <br />Section: 1.315 Page 3 of 4 <br />