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r 1 F Microbiological Parameter Analyses oct, 1 Routine evaluations to establish the relative numbers of heterotrophic (nonspecific) and selec- tive (contaminant-degrader) microbial populations at a site are performed initially during site characterization to aid in determining the feasibility of a desired bioremedial approach and, again, as a part of bioprocess monitoring activities following the selection and implementa- tion of the treatment method. During the treatment process, it is imperative that microbial populations be evaluated and tracked periodically to ensure that necessary growth require- ments are being maintained, and the desired biodegradative activity is occurring at an Opti- mum level. Microbial monitoring provides the necessary information that is required in order to establish the existence and maintenance of an effective proportion of selective (biodegrader) micro- organisms as compared to the overall general (heterotrophic) microbial population. In a sense, these two populations are supported by different carbon sources where the selective subpopulation utilizes the specific target contaminant (i.e., petroleum hydrocarbons) and the heterotrophic population metabolizes other alternative carbon sources (i-e., carbohydrates). It is this ratio (percentage) of selective to general population that allows one to track fluctua- r tions in the population of contaminant-degrading microbes, and, if necessary, adjust the treatment protocol to favor the growth and biodegradative capabilities of the selective mi- crobes. Microbiological monitoring is typically performed in conjunction with physicochemical (nutri- ent) and contaminant analyses and provides supporting data in determining the efficiency of biotreatment operations. LIn addition to site characterization and bioprocess monitoring evaluations, BioLogic performs custom microbiological assays for enumerating and/or characterizing specific microbial T_ groups, and supplemental microbial evaluations for bacterial identification of indigenous or ' exogenous commercial microbial products. Microbiological Analyses Conducted By BioLogic: 0 General(heterotrophic) Plate Counts 21 Selective (contaminant-specific) Plate Counts 2 Methanotrophs, Methanogens 0 Yeast, Molds and other Fungi 0 Physicochemical(nutrient) Analyses 10 Microaerophiles 0 Pathogen Screens 0 Bacterial Identification 3