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Pacific EcoRisk Environmental Consulting &Testing <br /> test solutions required adjustment to pH8.5 (see Section 2.2,above),and as an additional QA <br /> measure,a"pH Adjustment Control,consisting of Lab Water Control medium similarly adjusted <br /> to pH8.5,was also prepared and tested.There were two replicates at each test treatment,each <br /> replicate consisting of 9.2 L of media in a 10-L container.Water quality characteristics (pH and <br /> dissolved oxygen [D.O.]) were determined for the test solution in each test replicate prior to <br /> initiation of this test. <br /> The test was initiated with the random allocation of 10 juvenile fathead minnows into each <br /> replicate container.These containers were placed in a temperature-controlled room at 20°C, <br /> under fluorescent lighting on a 16L:8D photoperiod. <br /> Each day,each replicate container was examined,and the number of live fish in each was <br /> recorded.Routine water quality characteristics (temperature,pH,and D.O.) of the treatment <br /> waters were measured and recorded for each replicate container each day. <br /> After 96 (±2) hrs,the test was terminated and the number of live fish in each replicate container <br /> was determined.The fish in a randomly selected Lab Control treatment replicate were then <br /> euthanized and measured for total length (to the nearest 0.1 mm) and weighed(to the nearest <br /> 0.001 g).The resulting survival data for the waste treatments were evaluated to determine the <br /> LC50 using the CETIS®statistical software(TidePool Scientific,McKinleyville,CA). <br /> 3.RESULTS <br /> 3.1 Effects of the Waste Sample"13 F.A.Ground"on Fathead Minnows <br /> The results of this test are summarized in Table 1.The LC50 of this sample was>750 mg/L, <br /> which is greater than the Title 22 "Hazardous Waste"classification threshold of 500 mg/L; <br /> accordingly,this sample should not be considered"hazardous" with respect to fish toxicity.The <br /> test data and summary of statistics for this test are presented in Appendix B. <br /> Table 1.Results of the hazardous waste toxicity evaluation <br /> of the waste sample"#3 F. A.Ground". <br /> Waste Sample Treatment Level Mean % Survival <br /> Lab Control 100 <br /> 250 mg/L 100 <br /> 500 mg/L 100 <br /> 750 mg/L 100 <br /> Summary of Key Statistic <br /> LC50 = >750 mg/L <br /> Pas 2. L1�� <br />