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-LODI MEMORIAL HOSPITIIJ JULY 1985 <br />x . <br />_® A LAVE OPERATiiva PROCEDURE <br />Sterilization using the American Cyclomatic Control Autoclave will be <br />complete when autoclaving at: <br />Temperature: Ill °C <br />Pressure: 118 tbs./sq. inch <br />Sterilizing Time: 30 minutes for contaminated material <br />15 minutes for media preparation <br />!) All contaminated material will be autoclaved in double autoclave gags. <br />Media- in, flasks will. have cotton plugs in the f task -mouth and_-�,crew <br />capped tubes will have the ceps partially screwed on, but NOT tight to <br />seal the tube. <br />3) Each load will have a strip of Diack-tape attached to one of the articles <br />being autoclaved. Diaack strips turn black when subjected to 115 lbs. at <br />i20° C. <br />4) Fo l i ow the operating Instructions printed on the control panel: <br />a. Fast -exhaust for the Autoclave bag contents. <br />b. Slow -exhaust for media and other liquids. <br />5) Record in the Autoclaving log each time a run is made. (see attached <br />form). <br />6) Microbiology will insure that a Burg -1 -spore -control strip will be run <br />weekly. The test packets with recorded results will be kept in micro- <br />biology. <br />7) The microbiology section°is--responsible for the operation of the autoclave. <br />8) Maintenance will 8e performed by hospital maintenance department. <br />Revised Procedure 6/25/85 <br />REVIEWED: <br />